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A GAME CHANGER FOR THE AGRICULTURE INDUSTRY(ROS) ENGINEERED TO IMPROVE PROFITS AND SAFETY IN AGRICULTURE

JC-9465 is a new proprietary advanced
chemistry that generates ROS (Reactive
Oxygen Species). It is an aqueous chelation
of minerals/metals and oxychloride
compounds in a liquid form. Our mineral
oxychloride technology is a source of oxygen
energy that is effective against 99.9999% of
bacteria and viruses. By generating a high
concentration of hydroxyl radical ions and
other oxygen species, JC-9465 has been
proven to kill microorganisms in less than 10
seconds. Independent studies have proven
that JC-9465 effectively raises the oxidative
energy of water, lowering the impact of
microorganisms as well as improving the
yield in agricultural applications worldwide.

BENEFITS TO THE AGRICULTURAL INDUSTRY
We enhance your company’s position as a leader in the agricultural industry, and we help
Facilitate the supply/value chain from pre-harvest to post-harvest.

  • We eliminate microorganisms like Salmonella, Listeria and E.Coli with a 6-log reduction (much faster than the Industry standard, 3-log) with NO change in the characteristics of food/crops/taste.
  • Pathogens do NOT develop resistance to JC-9465.
  • Low-cost method to increase the oxidative energy for healing plant life in a sustainable and ecological manner.
  • Low-cost method to increase the dissolved oxygen in water which is a crucial factor in achieving healthy root and plant growth. Healthy roots with a good supply of oxygen have better aerobic respiration and are able to selectively absorb more ions in solution.
  • Improves yield of harvest with existing resources by increasing the oxidative energy in the root system and around the leaf and stem area.
  • Controlled ripening and Improved shelf life up to 2X by controlling biofilm on the surface
  • JC-9465 is USDA Organically certified for use as a crop production aid, disease and pest control aid, fertilizer and soil amendment aid, livestock production aid and processing, and handing aid for facilities requiring organic certification.
  • We eliminate biofilm from all surfaces in contact with water such as drip irrigation systems, reducing clogging, improving efficiency and lowering costly maintenance expenditures.
  • We use ROS to enhance the natural immune defense systems of plants and trees against pathogens, using less energy for survival, which allows them to use more for growth.
  • JC-9465 provides a residual protection that will continue to work and prevent bacterial growth in stagnant water.
  • JC-9465 can reduce issues associated with spoilage, cross contamination and pesticides.
  • Applications extend to sterilization and food preservation in post-harvest wash, packaging, storage and distribution, enhancing the supply chain performance.
  • US EPA Registered, NSF Certified and USDA Organic Certified.

HOW DOES JC-9465 WORK?
“An ecological and sustainable form of Liquid Ozone”

  • Mineral oxychloride generates a high concentration of hydroxyl radical ions and other oxygen species that kill microorganisms in less than 10 seconds.
  • It oxidizes and penetrates the cell membranes of microorganisms using available electrons, driving more energy and nutrients though the plants/trees xylem and phloem.
  • Enhances the mobility of nutrients from the roots system to the leaves.
  • Molecules are purposely designed to be weakly bound together such that when it comes in contact with inorganics, microorganisms and organic matter, it readily gives off oxygen atoms that aggressively oxidize all contaminants.
  • JC-9465 is catalyzed by the transition minerals’ internal vibrational energy, which activates the reactive oxygen species. In this very energy-efficient process, all of the generated power is created and used at the atomic level.
  • Hydroxyl radicals enhance the mineral oxychloride reagents’ electrochemical potential, and it is the strongest oxidant in the water treatment industry, estimated to be in the range of 2.8 mV to 2.90 mV.
  • The mineral oxychloride reagent is not linear in its pathway to oxidation. It is a continuous chain reaction that is autocatalyzed by the inherent catalysts. It will stay active and protect the water, and its residual oxidative energy will not diminish until a new contaminant is introduced into the system.
  • JC-9465 controls biofilm by diffusion and penetration into the biofilm, damaging the cell membrane and its DNA. The reactivity or ROS in contact with organics causes an etching effect which creates a chemical breakdown of the EPS matrix (Extracellular Polymeric Substances), making it unable to hold the content of the bacteria.
  • ORP refers to Oxidation Reduction Potential”as measured in millivolts, which can be easily monitored from a sample of water.
  • The following technologies have NOT been successful in removing biofilm: disinfection, flocculation, chlorination, coagulation, sedimentation, filtration, refining, UV irradiation and ozonation, irradiation with UV, ozonation and chlorination. They lack the ability to distribute sufficient oxidation energy (ORP) to control bacterial EPS and the AOC (Assimilable organic carbon) that feeds microbial colonies.
  • Simple low-cost application – Only requires a liquid dosing system with automatic ORP controller.

THE POWER OF JC-9465
Chemical Engineering for the 21st Century

JC-9465 is a new advanced oxidative technology for generating reactive oxygen species (ROS) that simulate an advance oxidation process (AOP) as a tool to measure disinfection effectiveness in water treatment solutions.

  • The World Health Organization
    recommends that water is safe at an
    ORP greater than +750 mV.
  • ORP is important in disinfection
    because it measures the energy
    available for disinfection, contact
    time required for disinfection and
    optimizes the treatment based on
    system demand.
  • JC-9465 is a low-cost producer of
    hydroxyl radical ions and is a very strong oxidant with a high concentration of oxidative energy; and it has a higher electronic footprint (Ev) vs a chemical
    footprint than other oxidants.
  • JC-9465 produces the same results as
    Ozone, but at a higher Ev. It generates a larger variety of highly reactive oxygen species in oxidative equilibrium, perpetually catalyzed by the vibrational energy of the electrolytes in solution.
  • JC-9465’s electrochemical potential is
    2.8-2.9 Ev. Ozone is 2.07 Ev and Sodium Hypochlorite is 0.94 Ev
  • Ozone is a well-known disinfectant for treating water. It can achieve a 6-log reduction in less than 10 seconds

JC 9465 is a new mineral oxychloride chemistry that is NSF approved, EPA registered as a biocide and USDA Organic Registered for use in applications requiring organic products only. In a study
conducted by Special Pathogen Lab (PA) using JC 9465, they confirmed that controlling the ORP
range above +700 mV effectively achieved a 6-log reduction of pathogens in less than 10 seconds.
JC 9465 is a mineral oxychloride chemistry that mimics nature’s ability to generate a very high
concentration of ROS in the form of hydroxyl radical ions and singlet oxygen ions. Please contact us
for more detailed information on this technology and how it can help you achieve better safety and
profits in your agricultural application.

Contact Us:
Jenfitch, Inc.
712 Bancroft Road Suite #805
Walnut Creek, CA 94598
Website: https://jenfitch.com
Phone: 1-925-289-3559
Fax: 1-925-289-0094
Email: Sales@Jenfitch.com

APPLICATIONS IN AGRICLUTURE :

JC-9465 is a transformative and revolutionary chemistry that has been successfully integrated into the
operations of many agricultural applications worldwide. The scope of applications extends from preharvest (such as fogging and irrigation), to sterilization and food preservation in post-harvest
applications such as washing, packaging, storage, and distribution. The following summarized
excerpts are a few examples of field studies, client reports, and internal/external independent research
studies. Please contact us for more detailed information on how JC-9465 can improve safety and
profits in your business.

JC-9465 Mineral Oxychloride rescues Citrus market in Florida
Working hand in hand with GreenAgri Solutions LLC, Jenfitch developed a treatment plan for an
infectious bacterium called huanglongbing (HLB) commonly known as “citrus greening”. The leaves of
newly affected citrus trees had developed blotchy mottle appearance and the fruit was of no value as it
had limited use in juicing operation. At the time, there had not been a commercially available solution to
this problem, except removing trees and re-planting several seasons later. The leaves of one test tree
were sprayed and the roots watered with a JC-9465 solution that generated an oxidized water having an
ORP (oxidation-reduction potential) greater than +700 mV. Infected leaves were removed and after a
week they started seeing a new “flush” of leaves on the infected tree. The test was expanded to 20 trees.
After 60 days of irrigating and spraying the trees with a +700 mV solution of the mineral oxychloride
solution, all the trees in the test started to develop marketable fruit.

ROS generated by JC-9465 is effective against Xylella fastidiosa Jenfitch was approached by an Olive grower in Europe as they had been struggling with a deadly tree-killing bacterium, Xylella fastidiosa, which has killed millions of olive trees in Southern Europe. Maria Saponari, a plant virologist at the Institute for “Sustainable Plant Protection” in Italy compares Xyelella to the coronavirus. Just as COVID-19 keeps oxygen from reaching our vital organs, she says, Xyelella attacks and clogs the xylem tubes of olive trees by forming a biofilm, so they can’t absorb water. Xylella spreads quickly and once the bacteria infiltrate the host, there is no cure for it, until now.

As per EU regulation, Xyelella Fastidiosa is considered a quarantine pest, which when detected must be contained or eradicated. A test solution of JC-9465 was prepared and applied to a small test site
implemented in fogging and root irrigation, effectively destroying and controlling the biofilm that blocks
the movement of water and nutrients in the host. By generating hydroxyl radical ions, JC-9465 breaks
down the extra-polymeric substance (EPS) which forms a sticky substance that acts as a protective film
around bacteria. Once the EPS is destroyed, other oxidative species quickly attacks the DNA of the
bacteria’s nucleus to inactivated and prevent reproduction or mutation. Albeit early, the results
reported have been extremely positive with leaves turning green, branches and twigs showing signs of
recovery and returning to normal production with good numbers. The tests have been expanded to a
larger sites and preliminary PCR results are very promising.

M&R Farms uses JC-9465 as a primary solution for their packing facility

M&R Farms in Birmingham, Minnesota, uses JC-9465 as a primary solution for their packing facility. They
have also incorporated JC-9465 into their ice-making machines, improving product shelf-life as well as
eliminating biofilm. By injecting JC-9465 into their water lines supplying the ice machines, the ORP
readings went up from 451 to 632. Once it had been converted to ice, the readings had increased to 783
ORP, destroying virtually all pathogens, such as Listeria and Salmonella. Furthermore, when the ice
melted, it continued to have the disinfecting power of JC-9465, as measured by ORP. Since
incorporating JC-9465, their ice is much flakier, the machines are running cleaner and producing more
ice daily. By removing pathogens and controlling biofilm with JC-9465, M&R Farms was able to improve
safety and extendthe shipping and shelf life of produce, seafood, and meat products.

Independent studies at U.C. Davis Post-Harvest Department, confirms that JC-9465 effectively raises the ORP to +700 mV inactivating E.Coli, Salmonella, and Listeria in less than 10 seconds and improving shelf life up to 2X. JC-9465 was tested and evaluated by one of the leading and well-known plant pathologists in the US for safety in controlling pathogens such as E.Coli, Salmonella and Listeria, as well as its effectiveness in controlling biofilm, that many food researchers believe is the source of contamination and spoilage. Theresults are shown below :

A poultry farmer in Montana contacted Jenfitch after reviewing the studies highlighting the
effectiveness of JC-9465 in eliminating Salmonella, Listeria monocytogenes and other pathogens, as
well as the effectiveness and lower cost of JC-9465 as compared to Sodium Hypochlorite, UV and
Ozone. Their main concern was decreasing the bacterial load on the shell surface of eggs and
preventing the infection of eggs through horizontal transmission, while still maintaining their USDA
“Certified Organic” assurance. Application of JC-9465 in their egg washing operation produced cleaner
eggs, with less breakage and eliminated the microorganisms with a lower contact time than the
previous sanitizing compounds. Furthermore, they were able to adjust the temperature of the water and
still produce a microbiologically safe egg, while saving energy costs associated with water heating.
Recent feedback has shown that they were able to improve cost-effectiveness in the pasteurization
process as well as increase sales of eggs sold to local hatcheries with better hatching results and
healthier chicks

Walking Strong: A Canadian dairy farmer uses JC-9465 as a primary preventative treatment in
their hoof care protocol.

Lameness and reduced mobility in dairy cows, caused by foot rot, heel erosions, sole ulcers and digital
dermatitis have led to body weight loss, decreased milk production, lower longevity and reproductive
efficiency. Concerned with the toxicity of formalin and copper sulfate, as well as the high cost of
antibiotics lead to the search for an alternative treatment. A “pre-footbath” area was used to minimize
organic matter. A trial with a 1 gallon of JC-9465 solution per 50 gallons of water was applied to the 10”
inch high footbath area along the exit lanes of the milking parlor, where the cattle passed several times a
day. A system was put in place to monitor the ORP of the water as measured in mV, in effect to monitor
the oxidative power (energy available for disinfection) of the solution, as well as an aid to determine the
frequency of solution replenishment. Results have shown a marked improvement in hygiene conditions
of hooves, lower bacterial count and less reported cases of digital dermatitis. Visual inspections have
shown to lower interdigital inflammation after a few passes through the foot bath.

The #1 Ranked Plant Sciences Reseach facility in the US evaluates JC-9465 as an
antimicrobial process aid for produce wash – Avocados.

A cocktail of Listeria Monocytogenes (PTVS 334 +335) was prepared and avocados inoculated.
Trial #1 : 100 Ul of Log-7 CFU/mL (Anticipated log4/fruit after drying.
Trial #2 : 100 Ul of Log-8 CFU/mL (Anticipated log 5/fruit after drying)
Once inoculated, avocados were allowed to dry at room temperature for 4 hours while covered
with cheese cloth before being kept at 10 °C for 3 days. For each trial, 15 uninoculated avocados were
submerged with 5 inoculated avocados in a mixture of 10L of water and JC-9465 at 4 °C for 30 seconds
with mild agitation. This was done twice per trial with different disinfectant doses.
A control wash was performed for each treatment by submerging 5 inoculated avocados in 10L of
water at 4 °C with mild agitation, and removed after 30 seconds. (This served as a control for
water-only detachment of rind contamination).
All avocados were massaged vigorously by hand with 20ml of potassium phosphate buffer + Tween
for 1 min and fruit discarded.
For mixed avocados (uninoculated and inoculated) , 20 ml(± fruit-adhering liquid) wash-buffer was
enriched with 20 ml of 2X lEB followed by 48 hr incubation at 37°C. Following incubation, 50 µI of
each sample bag was spot platted on CH ROM agar Listeria +Rif then incubated for 48 hr at 37°C
For inoculated avocados, 2 ml of wash-buffer was extracted for quantification and the remaining 18ml
of 2X lEB was added to each sample bag. Enrichment procedure as previously described with the
mixed avocados. Quantification was done by concentrating, direct and dilution plating on Chromagar
Listeria+ Rif then incubation for 48 hr at 37°C.
Sodium hypochlorite was added to the wash water from inoculated fruit to obtain a final solution of
20-40PPM of free chlorine at pH 7.0
10 ml of water was sampled at 0, 5, 15, 30, 45, 60, and 300 seconds and placed in Whirl-pak bags
withholding neutralizing buffer until each time-point extraction.
Water samples taken from the control wash treatment, were added to a bag along with 10ml of 2X
LEB. followed by 48 hr incubation at 37°C.
Following incubation, 50 µI of each bag was spot platted on CHROMagar Listeria +Rif then incubated
for 48 hr at 29 C to increase sub-lethal injury resuscitation.

A Research Study conducted by Frontiers in Microbiology Research – Evaluation of JC-9465
and Neutral Electrolyzed Water in Controlling Listeria monocytogenes on Fresh Apples and
Preventing Cross-Contamination

Recent multistate outbreaks and recalls of fresh apples due to Listeria monocytogenes contamination
have increased consumer concerns regarding fresh and processed apple safety. This study aimed to
evaluate the antimicrobial efficacy of two sanitizers, mineral oxychloride (JC-9465) and neutral
electrolyzed water (NEW), for inactivation of L. monocytogenes on fresh apples. A 2-min treatment of
0.125% (v/v) JC-9465 with 100 ppm free available chlorine (FAC) or NEW with 110 ppm FAC caused
0.9-1.2 log10 CFU apple reduction of L. monocytogenes on both Granny Smith and Fuji apples 24 h
post-inoculation. IIncreasing JC-9465 concentration to 0.25 and 0.50% significantly improved its
bactericidal effect and reduced L. monocytogenes on Granny Smith apples by ~2.0 and 3.8 log10 CFU/
apple, respectively, after a contact time of 2 min. At a shorter contact time of 30 sec, the inactivation
efficacy of chlorine and 0.25–0.50% JC-9465 against L. monocytogenes on apples was significantly
reduced compared with the respective 2-min wash. Furthermore, no L. monocytogenes was recovered in
deionized water prepared antimicrobial wash solution or on non-inoculated apples post-NEW with 110
ppm FAC or 0.125 – 0.5% JC-9465 washes, indicating their ability to prevent cross-contamination. In
addition, a 2-min exposure to NEW with 110 ppm FAC and 0.50% JC-9465 reduced apple native
microbiota including total plate count by 0.14 and 0.65 log10 CFU/apple, respectively, and yeast and
mold counts by 0.55 and 1.63 log10 CFU/apple, respectively. In summary, L. monocytogenes attached on
apples was difficult to eliminate. JC-9465 and NEW demonstrated a dose- dependent reduction in L.
monocytogenes on apples and successfully prevented cross-contamination, indicating their application
potential in post-harvest washes of apples.

A rice farmer from South East Asia tests JC-9465 to control Bakanae disease, caused by Fusarium fujikuroi, a seed-borne fungus.

Suffering from approximately 30% loss in yield, a paddy farmer contacted Jenfitch to test JC-9465 as an alternative treatment for chemical
seed dressing. Existing treatments such as thermo-therapy and aqueus suspension containing fungicides have produced mixed results.
Thermal effect do not reach the pericarp of severely infected seeds, and fungicide treatments do not work well for destroying the spores of this
fungal pathogen. Furthermore some pathogens have showed resistance to fungicides. Although Bakanae is a seed-borne fungus, it may
occur when the pathogen is present in plant material or soil. Infected seeds/plants result in secondary infections, which is spread through
wind and water. The flooding of rice fields also decreases the soil reduction-oxidation (redox) value which intensifies emissions of
greenhouse gasses and increases the plant mobility of Arsenic (As) to rice. Increased exposure to toxic inorganic arsenic iAs is a
public health risk, which is particularly concerning regarding the iAs content of rice-based baby foods. (Talk to Charles about this
and JC-9465).

In a study conducted by the Department of Plant Pathology and Crop Physiology,
Louisiana State University Agricultural Center, Baton Rouge, concluded that “Bacteria were completely eliminated from rice seeds following immersion in a Sodium
Hypochlorite solution adjusted to pH 7.0 in 0.5 M potassium phosphate, while fungi
were eliminated at pH 5.0 and below. Sodium hypochlorite stimulated rice seedling growth directly, as opposed to an indirect effect related to elimination of microbial contaminants or alleviation of dormancy”. Although JC-9465 had not been tested directly in rice farming prior to this, our proprietary mineral oxychloride has a higher electronic footprint (Ev) vs a chemical footprint than other oxidants. The test results through customer feedback prove that a higher concentration of oxidative energy produced by JC-9465 was more effective than their existing treatments. JC-9465 effectively suppressed horizontal transmission of the pathogen between seedlings. Visible Bakanae symptoms, of altered morphology improved significantly and loss in yields have been reduced to below 10%. Tests have been expanded to incorporate spraying of the rice flowers and treatment of wastewater for paddy field flooding.

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